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Gram stain reagents

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  Gram stain reagents

These stains can be ordered as a complete kit from VWR (#l5204-004) or can be reconstituted as follows:

 Image result for gram stain reagents
Crystal violet
crystal violet (90% dye content)                            20.0 g
ethanol (95%)                                                         200 mL
ammonium oxalate                                                 8.0 g
dH20                                                                       800 mL



Mix  first  two  ingredients  and  let  sit  overnight  or  until  dye  goes into solution.     Add remaining ingredients and filter before use.

Gram's iodine
iodine crystals                                                        1.0 g
potassium iodide                                                    2.0 g
dH20                                                                       300 mL
                    
Decolorizer
acetone                                                                   40 mL
ethanol (95%)                                                         60 mL

                    
Safranin
safranin O                                                               2.5 g
95% ethanol                                                           100 mL
dH20                                                                       900 mL
Filter safranin solution before use.





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