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Glucose blood test / Methods GOD-POD

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Specimen: 
Serum patient
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Equipment :
Tube 4, Micropipette, micropipette tip, GOD-POD reagent , Solution control and standard .watch,Bain-marie,spectrophotometer,....
Testing
pipette into well identified test tubes:

  1. Bank: reagent 1ml + demineralised water 0.01ml
  2. standard : reagent 1ml + solution standard 0.01ml 
  3. control :   reagent 1ml + serum control 0.01ml
  4. serum patient : reagent 1ml + serum patient 0.01ml

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Then take incubation 15mn temperature 37c
 Read absorbance at (460-560) nm  with spectrophotometer
Calculation 
Result =  Abs (control)/Abs (standard)*standard concentration (follow via reagent kit) for QC testing
Result =  Abs (serum patient)/Abs (standard)*standard concentration (follow via reagent kit)
 Result : Glucose: ........mg/dl
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