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Mueller Hinton Agar

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Mueller Hinton Agar (MHA)       The major use of Mueller Hinton Agar is for antimicrobial susceptibility testing. Composition of MHA Ingredients   Beef Extract                                  2.00 gm Acid Hydrolysate of Casein         17.50 gm Starch                                           1.50 gm Agar                                             17.00 gm Distilled Water                             1000 ml Final pH 7.3 ± 0.1 at 25ºC Why Mueller Hinton agar is used for antibiotic susceptibility tes...
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Urease Test

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Urease Test The urease test is used to determine the ability of an organism to split urea, through the production of the enzyme urease. Principle  Urea  is the product of decarboxylation of  amino acids . Hydrolysis of  urea  produces  ammonia  and  CO2 . The formation of  ammonia  alkalinizes the medium, and the pH shift is detected by the color change of  phenol red  from  light orange  at pH 6.8 to  magenta (pink)  at pH 8.1. Rapid urease-positive organisms turn the entire medium  pink  within 24 hours. Weakly positive organisms may take several days, and negative organisms produce  no color change  or  yellow  as a result of  acid production . Uses  1.     This test is used to differentiate organisms based on their ability to hydrolyze urea with the enzyme...
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INTRODUCTION TO MYCOLOGY

By ok learning
MYCOLOGY INTRODUCTION TO MYCOLOGY Contents 1 Objective 2 Meaning 3 Growth 4 General structures FUNGI: 1 Purpose · Types of colors and definitions · Separation of yeast and mold 2.Definition Mycology is a fossil science study. Mycosis is a fungal infection. Yeast is a large cell, with only one type of cells, most of which are non-transplantation, called budding. Mold is a multicellular cell. Most of the fertilized egg is enlarged. 3 Growth a.Yeast: routine incubation temperature is usually 25c b. Mold: routine incubation temperature is usually 25c o to 30c o , although 35c o incubation can be used to differentiate some mold based on temperature tolerance or determine whether organisms are diphasic. c. Diphasic (dimorphic fungi): These organisms differ in two forms depending on temperature. d. Frequently used media The most common use of: - spermatozoa-Sabouraud's dextrose agar (SDA) - Sabouraud's dextrose agar ...
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