INTRODUCTION TO MYCOLOGY

Contents

1 Objective

2 Meaning

3 Growth

4 General structures

FUNGI:

1 Purpose

· Types of colors and definitions

· Separation of yeast and mold

2.Definition

Mycology is a fossil science study.
Mycosis is a fungal infection.
Yeast is a large cell, with only one type of cells, most of which are non-transplantation, called budding.
Mold is a multicellular cell. Most of the fertilized egg is enlarged.

3 Growth

a.Yeast: routine incubation temperature is usually 25c

b. Mold: routine incubation temperature is usually 25co to 30co, although 35co incubation can be used to differentiate some mold based on temperature tolerance or determine whether organisms are diphasic.

c. Diphasic (dimorphic fungi): These organisms differ in two forms depending on temperature.

d. Frequently used media The most common use of:

- spermatozoa-Sabouraud's dextrose agar (SDA)

- Sabouraud's dextrose agar with antibiotics (Mycosel)

- Brain-heart infusion agar (BHIA)

4 General structures:

Type of:

yeast-Illustration of

-Illustration of blastoconidia

-Illustration of pseudohyphae

-Illustration of true hyphae

-Illustration of a germ tube

Ø Sexual reproduction: Ascospores occur in a special bag or basidiospores occurring at the bottom of a club-shaped structure called basidium.

Ø Asexual reproduction: blastoconidia, arthroconidia, pseudohyphase and true hyphase.

Type of Mold:

-Illustration of sporangium and sporangiophore

-Illustration of conidia and conidiophore.

Comments

Microscopic examination of urine

Microscopic examination of urine expressing microscopically observed leucocytes, erythrocytes, and casts in urine, both with centrifugation, Testing Specimen: Urine patient Equipment: tube, centrifuge,timer,pipette transfer,microscope side, cover slip,microscopy Testing: Urine take in tube 3/4 of tube Take to centrifuge 3000rmp/1mn at 5 minutes Decant out of above liquid Pipette transfer sucks sediment take on microscope side and cover slip Examination with microscopic Result: Reference: www.time2026end.com Laboratory book livre de lavoratorie 1th edition 1999 https://www.google.com/url?sa=i&url=http%3A%2F%2Fe-learning.studmed.unibe.ch%2FUroSurf_EN%2Ftheory%2Fsedimethods2.html%3Furosurf%7Ctheory%7Csediment%7Csedimethods%7C2&psig=AOvVaw0KIQHg49jHVyHFdvza_3TL&ust=1582250323839000&source=images&cd=vfe&ved=0CAMQjB1qFwoTCNDK8fmD3-cCFQAAAAAdAAAAABAg

MacConkey Agar

MacConkey Agar MacConkey agar is a selective and differential media used for the isolation and differentiation of non-fastidious gram-negative rods, particularly members of the family Enterobacteriaceae . Composition MacConkey agar is used for the isolation of gram-negative enteric bacteria and the differentiation of lactose fermenting from lactose non-fermenting gram-negative bacteria. Pancreatic digest of gelatin and peptones (meat and casein) provide the essential nutrients, vitamins and nitrogenous factors required for growth of microorganisms. Lactose monohydrate is the fermentable source of carbohydrate. The selective action of this medium is attributed to crystal violet and bile salts, which are inhibitory to most species of gram-positive bacteria. Sodium chloride maintains the osmotic balance in the medium. ...

Triple Sugar Iron Test

The Triple Sugar Iron (TSI) Test To determine the ability of an organism to ferment glucose, lactose, and sucrose, and their ability to produce hydrogen sulfide. Principle The Triple Sugar Iron (TSI) test is a microbiological test named for its ability to test a microorganism’s ability to ferment sugars and to produce hydrogen sulfide. An agar slant of a special medium with multiple sugars constituting a pH-sensitive dye (phenol red), 1% lactose, 1% sucrose, 0.1% glucose, as well as sodium thiosulfate and ferrous sulfate or ferrous ammonium sulfate is used for carrying out the test. . Due to the building of acid during fermentation, the pH falls. The acid base indicator Phenol red. is incorporated for detecting carbohydrate fermentation that is indicated by the change in color of the carbohydrate medium from orange red to yellow in the pres...

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